This white paper provides an overview of the molecular probes available to the cell biologist and how they have been applied in different fields, going over specific examples from modulation of intracellular signaling pathways to organelle positioning dynamics.
It also compares common patterned illumination technologies that cell biologists are familiar with: galvo-based scanning and digital micromirror device (DMD) systems.
Are you interested in utilizing patterned illumination in your neuroscience research and deciding what tool is right for you?
This paper will discuss several techniques of patterned illumination and their relative merits in an experimental setting, specifically the use of direct projection systems such as digital micromirror devices (DMD), holographic projection systems, and galvo-based scanning systems in combination with optogenetic constructs. Each of these techniques has its own advantages and drawbacks.
Are you interested in all-optical methods for probing in vivo neural activity and deciding what tool is right for you?
This paper will discuss several tools that enable in vivo calcium imaging and optogenetics and their relative merits in an experimental setting. Specifically, the advantages and drawbacks of two-photon imaging, head-mounted microscopes, fiber photometry, and fiberscopes for in vivo imaging and optogenetics will be discussed.
by James Butler, Department of Neuroscience, University of Cambridge, UK
This application note reports on how the Polygon400 was used to provide optogenetic stimulation of specific layers of the CA1 with oscillating temporal patterns in mice brain slices.
by Jacob Robinson, Rice University, USA
This application note reports on a open-source patterned illumination software developed out of Rice University that uses the Polygon400.
This application note reports on how the Polygon400 was used on the OASIS Micro for targeted optogenetic stimulation to decode olfactory codes in head-fixed mice.